1. The role of each structure fermenter

1.1 precision filter is generally used for a period of one year. If the filter is too much resistance or loss of ability to filter  induced normal production, you need to be cleaned or replaced  (recommended direct replacement, no cleaning, because after cleaning  operations can not reliably guarantee performance of the filter).

1.2 cleaning fermentors, scrub with a soft brush, do not scratch hard to avoid any surface damage fermenter.

1.3 supporting instruments should be checked every year to ensure proper use.

1.4 electrical, instrumentation, sensors and other electrical  equipment is prohibited directly with water vapor contact, to prevent  moisture.

1.5 devices to stop using, should be promptly cleaned, drained  fermenter various pipeline than water; loosen the fermentation jars and  hand hole cover screw seal to prevent permanent deformation.

1.6 operating platform, heated tanks and other steel equipment should be regularly (once a year) paint to prevent corrosion.

1.7 If the fermenter temporarily do, you need to eliminate the  fermenter were empty, and all side by side to make more than the water  tank inside the tubes.

2. Considerations

2.1 single piece of equipment must ensure that all use of the system can run normally.

2.2 sterilization filter, air filter flows through the steam pressure  should not exceed 0.17MPa, otherwise the filter cartridge will be  damaged, lost filtering ability.

2.3 During the fermentation process, make sure the tank pressure does not exceed 0.17MPa.

2.4 In the real process of elimination, when the jacket through the  steam preheat inlet pressure must be controlled within the operating  pressure range of the device (no more than 0.2MPa), otherwise it will  cause damage to the fermenter.

2.5 Cancellation and empty real consumer, must be drained fermenter jacket remainder water. Doing so may lead to the fermentation tank flattened cylinder,  resulting in equipment damage; when the real consumer, but also cause  excessive condensate leads broth diluted, making it impossible to  process requirements.

2.6 After the air in the cooling process elimination, elimination of  solid, non-fermentation tank to produce a negative pressure to prevent  pollution, and even damage the equipment.

2.7 in the fermentation process, the pressure tank should be maintained between 0.03 ~ 0.05MPa, in order to avoid contamination.

2.8 In each procedure, you must keep the air pressure in the pipeline  pressure is greater than the fermenter tank, otherwise it will cause  fermentation liquid back into the filter, clogged filter cartridge or  the filter failure.

2.9 If you encounter a problem they can not solve, please contact the company service department. Do not force the demolition or repair.

Various parts of the structure:

Three-part structure: tank and control box, air compressor (Air  Station), steam generator (boiler room), mainly on the tank and the  control box.

To a stainless steel tank, volume of 30 L,

Tank  Structure: The main material is stainless steel 316L tank, the tank no  dead ends; seed fermenter with a dedicated sampling pipes, discharge  valve, shift. Using  a large tank level perspective endoscopic observation, 12V safety  depends on light, with temperature, PH, DO, tank top with an exhaust  port, a pressure gauge, a safety valve, a foam sensor interface,  bacteria inoculated mouth 1, an anti-foaming liquid interface, acid (base) port 1, port 2 feeding, depending on the cap 1

Tank-side interface: air intake, a sampling valve interface, temperature, PH, DO interface for each one.

Tank bottom interface: a discharge port.

Sampling valve, no dead discharge valve design, can be sterilized separately

Tank: Design pressure 0.3Mpa jacket: design pressure 0.35Mpa, optimized diversion design

All  metal parts in contact with the material, pipe materials are stainless  steel, non-metallic parts require high temperature, corrosion,  non-toxic, environmentally friendly, needle valve, ball valve and  diaphragm (standard diaphragm, high temperature, acid) can tank Different configurations of the controller performance. The controller can complete the most basic functions, which consists of the following parts:

(1) Parameter Input and display means for control of the fermentation  conditions of the various input parameters and display the culture  medium during the fermentation tank temperature, pH, DO (dissolved  oxygen) measured value.

(2) the electrode correction means for correcting the pH electrode and DO electrodes.

(3) an acid, a base is added to the fermentor pump for acid or alkali to adjust the culture medium of pH.

(4) a defoaming agent was added to the pump defoamer was added to the  fermentor in order to eliminate the excessive foam during fermentation.

(5)  warning light and buzzer button when the fermentation process, the  failure on the circuit, such as the display shows the temperature or DO  is flashing, that exceed the measured value of the unit, the alarm red  light and issued a "tick, ...... tick "sound.

(6) Automatic or manual control button is in the controller for determining the present automatic control or manual control.

(7) electrically connected to the wire: connection wires are connected with pH, and temperature probe.

Fermenter using mechanical operation

Installed electrodes. Check before sterilization each interface, each line appearance is normal, the steam pipes are secure. They should check whether each valve is in a fully closed state.

Sterilization

Requires at least two people present during sterilization, a person  responsible for the operation of various valves, one to observe the  parameters of the report.

After  confirming that each valve is closed, open the steam generator inlet  valve, start the steam generator, steam generator automatically starts  the water, you should open the lower part of the steam generator drain  valve to the water as possible after release rust close the drain valve . Observe the water level inside the steam generator, to prevent accidents. At  this point the valve is fully open valve jacket, slowly open the steam  generator of the total valve partially open jacket inlet valve slowly,  began to pass into the steam jacket. Vapor  pressure at the start may instability, violent swing gauge pointer can  be appropriately small jacket off the intake valve, then open the steam  valve sampling valve, sampling valve into the steam valve, foot valve  out of the steam valve, foot valve into the steam valve for sampling valve and bottom valve sterilization, while water vapor pressure instability drained. No dramatic changes in water vapor pressure when in turn closes the valve bottom valve. Tank  temperature gradually rises to 90 ° when the set temperature, stirring  motor is stopped, the tank total intake valve opens, open the tank  outlet pipe valve, open the following valves: open outlet valve, slowly  open the tank into  the steam valve to the tank through the steam, air filter gauge begins  to rise, the valve opens hydrophobic condensate exceeds 0.1MP, continue  to open a large tank into the steam valve, after divisible air tank, the  tank pressure to rise slowly, adjusting outlet  valve, so as to achieve the desired sterilization temperature and  pressure, the start time is reached sterilization temperature. Adjust  the jacket inlet valve, the tank into the steam valve and outlet valve,  the temperature, pressure tank to maintain the set temperature. Sterilization  time to post, and then click Close has opened each inlet valve, close  the main steam valve fully open tank exhaust vent let go of the pressure  tank, turn off the steam generator power. Open the air compressor, etc. can pressure when dropped 0.05MP, slowly  open the intake valve, maintaining a positive pressure inside the tank.

Sterilization process, the operator should wear long trousers with protective gloves to prevent burns. Sterilization  process if automatic water steam generator, steam total pressure will  drop quickly, if necessary, appropriate to open a large main steam  valve, keep the pressure, but be careful observation of the pressure  change. When the tank through steam sterilized jar pressure decreased rapidly, attention through sterile air time. Try not to make air compressors and steam generators simultaneously. Air filter pressure sterilization process should not exceed 0.2MP. Will affect the size of the condensate tank after sterilization liquid  concentration, to consider increasing the amount of steam condensate.

Ferment

Control  of the intake valve exhaust valve can be resized and tank pressure  ventilation, control agitation revolutions fermentation dissolved oxygen  to meet process requirements. Open  fermentation tank inlet valve, open the valve and jacket jacket inlet  valve, then the system automatically by controlling the jacket  temperature control of fermentation temperature. Due  to the positive pressure tank, when inoculated off a small inlet valve,  due to lower tank pressure to 0.01-0.02MP, flame biosphere inoculated  mouth quickly open (to prevent air flow to the flame goes out), after  inoculation inoculation mouth shut quickly. Will  be linked into the bottle after each feeding systems and ancillary hose  sterilization host (note the direction), pierce silicone mat under fire  ring protection installed feeding system. You should have a system to display real-time monitoring of various parameters on the workstation. Note that parameter records do work.

Fermentation  late, you may have a bacterial blocked out the trachea, the phenomenon  of decreased ventilation, tank pressure slightly increased. At this point man rotation around the valve, you can make jam eased.


FAQs and Troubleshooting

Temperature control failure

Possible Cause: The total tank valve is not open Solution: open a large tank ventilation valve total decline

Possible causes: bacterial clogging the trachea resolved: man rotation vent abnormally high pressure air filter

Possible causes: Air Filters Memory Water Solution: Open the filter traps

Emergency treatment

Power failure

First, turn off the air compressor to prevent sudden power transmission. Turn off the host control unit and the host. Try to maintain the tank pressure and temperature. After power is restored, turn on the host, the control unit and  workstations, to be reduced to zero open air compressor pressure air  compressor

Sterilization process tailpipe thrown or broken line interface

Trying to close the steam valve, turn off the steam generator, turn off the power. Security personnel evacuated zone, re-cooled exhaust pipe is fixed or replaced accordingly pipelines.